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KMID : 1039420150490010023
Journal of Pathology and Translational Medicine
2015 Volume.49 No. 1 p.23 ~ p.29
PHH3 as an Ancillary Mitotic Marker in Gastrointestinal Stromal Tumors
Shin Yoo-Ju

Hyeon Ji-Yeon
Lee Bo-Ram
Ha Sang-Yun
Hong Min-Eui
Do In-Gu
Kim Kyoung-Mee
Abstract
Background: Counting mitoses is subjective and time-consuming. The adjunctive diagnostic utility of a recently reported mitotic marker, phosphohistone H3 (PHH3), was investigated in gastrointestinal stromal tumors (GISTs).

Methods: We reviewed 77 GISTs for several proliferative indices. These included the mitotic count per 50 high power fields (HPFs), the immunohistochemical Ki- 67 labeling index and the immunohistochemical PHH3 mitotic index (MI). For comparison, Spearman¡¯s rank correlation and interclass correlation coefficient were used.

Results: Mitotic counts ranged from 0?138 (mean, 7.57¡¾2.34) and the PHH3 MI ranged from 0?126 per 50 HPFs (mean, 9.61¡¾2.27). We found a positive correlation between mitotic counts and PHH3 MI (r=0.810, p<.001). The inter-observer correlation coefficient for three participants was 0.975 for mitotic counts and 0.940 for the PHH3 MI. When using the PHH3 MI instead of mitotic counts in the Armed Forces Institute of Pathology (AFIP) stratification criteria, 10 cases were reclassified. In one patient with a mitotic count of 2 and a PHH3 MI of 6 per 50 HPFs, distant metastasis occurred.

Conclusions: In GISTs, the PHH3 MI correlated adequately with mitotic counts and can be used as a useful adjunctive to count mitotic figures efficiently.
KEYWORD
Gastrointestinal stromal tumors, Mitosis, Ki-67, Biological marker
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